Science-backed algorithms

PepCheck

Comprehensive physicochemical analysis of protein and peptide sequences

30+ Parameters
Scientific References
Peer-reviewed Methods

Paste FASTA format or raw amino acid sequence

Computed metrics: Molecular weight, pI, GRAVY, aliphatic index, instability index, extinction coefficients, N-end rule half-life, net charge, hydrophobic moment, aromaticity, aggregation & solubility heuristics, and more.

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Accurate Calculations

Uses Bjellqvist pKa sets, Guruprasad DIWV, and validated hydropathy scales

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Scientific References

Every metric links to primary literature (Kyte-Doolittle, Ikai, etc.)

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Export & Share

Shareable result URLs for reproducible analysis